FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_15_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_15_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2599013
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT164590.6332788639379641No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT148430.571101414267647No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT127990.4924561747093993No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT120250.4626756387905716No Hit
GAAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAAC112680.43354919732990943No Hit
CACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA98020.3771431693492876No Hit
CACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA97030.3733340310340887No Hit
AAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACA95130.36602356356047466No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT88800.3416681640299606No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC80370.3092327741338731No Hit
CTCTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT76670.2949966006326248No Hit
ATGTTATATTGGTCTACTGTTGTTTGCTTCAAAACTGTTTTGGCCTCATT74940.2883402276171762No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT72890.2804526179745927No Hit
ATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGT72880.2804141418299947No Hit
CATCTTGCTTTATCAAGGAAAACCAGTTAAAGTAGTGAAAAGGAAAAGGT58960.22685534854962247No Hit
AGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACAA55770.21458145842287052No Hit
GGATGAAGTGGGCCATGTGTTCTAACTTTCCCTTGGCTCTACCCAGATCT55010.21165727143342492No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT52370.20149956925956122No Hit
ACCCAGATCTTGGAGATTTCAAAACTATTGAAGAACTTGAAAAGCTGAAA49540.19061082033833612No Hit
GTAGTAACAAGAGGATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT47480.1826847345511546No Hit
GAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAAC46540.17906797695894555No Hit
AACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATT45810.17625921840329387No Hit
AATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTG45010.1731811268354564No Hit
GTATAGTGCTTTGTCCAATGACATTTCACAAGGAATTAAGAGACAAAGAA44940.1729117938232706No Hit
GTTAAAACAACTGCTAAGGGACAATGAGGCCAAAACAGTTTTGAAGCAAA44140.16983370225543312No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT43690.16810227574852454No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT43520.16744818129035907No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG40990.15771371670707302No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG40980.15767524056247506No Hit
GCTAAGGGACAATGAGGCCAAAACAGTTTTGAAGCAAACAACAGTAGACC40550.15602076634476242No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT40170.1545586728500396No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC39970.15378914995808024No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT39180.15074953453484072No Hit
GCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTC38730.14901810802793214No Hit
ATTCAATACATCAAGGATTGAAAAGAATCCTTCACTAAGGATGAAGTGGG38010.1462478256168784No Hit
GTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT37850.14563220730331092No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT37640.14482420826675357No Hit
TCATTGGACAAAGCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTT37030.14247716344627748No Hit
GAATTAAGAGACAAAGAATGACAGTTGAGTCTATGGGGTGGGCCTTGAGC36350.13986078561361565No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT35620.13705202705796393No Hit
TCTTAATTCCTTGTGAAATGTCATTGGACAAAGCACTATACCTTTTCCTT35620.13705202705796393No Hit
GACCAATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAA35550.13678269404577814No Hit
GCTCTACCCAGATCTTGGAGATTTCAAAACTATTGAAGAACTTGAAAAGC35070.13493583910507567No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT34680.1334352694657549No Hit
ACTTTAACTGGTTTTCCTTGATAAAGCAAGATGTTTGCCTTTTCCCCTGG34520.13281965115218738No Hit
CTTAATTCCTTGTGAAATGTCATTGGACAAAGCACTATACCTTTTCCTTT33980.13074193934389708No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA33660.1295107027167621No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA30730.11823719234955732No Hit
AGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACAA30610.1177754786143817No Hit
GTTCTAACTTTCCCTTGGCTCTACCCAGATCTTGGAGATTTCAAAACTAT30180.11612100439666904No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA28730.11054196342996361No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT28690.11038805885157173No Hit
GTCCAATGACATTTCACAAGGAATTAAGAGACAAAGAATGACAGTTGAGT28520.10973396439340627No Hit
ACATTAGCCAAAATTGAATTGTTAAAACAACTGCTAAGGGACAATGAGGC27870.10723301499453831No Hit
GAATTAATGGATAAATTTATATTAGCTCAAGGCCCACCCCATAGACTCAA27700.10657892053637286No Hit
CATCAAGGATTGAAAAGAATCCTTCACTAAGGATGAAGTGGGCCATGTGT27600.10619415909039315No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG27320.10511682704165005No Hit
GGTTTCAGCTTTTCAAGTTCTTCAATAGTTTTGAAATCTCCAAGATCTGG27100.10427035186049473No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT26880.10342387667933942No Hit
CAATTGCGTTCATTGAATTAATGGATAAATTTATATTAGCTCAAGGCCCA26810.10315454366715364No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA26510.1020002593292146No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAT100.007090031145.010123
TCCCGGT100.007090031145.010122
TTACACG100.0070937024144.9858
AGCACGC16700.0126.365471
GTAACAA68250.0126.100374
GCACGCA17400.0121.258462
CACGCAC17550.0120.222063
GAAACAC32000.0118.953614
TAACAAG73550.0117.605095
CGGTGCA458.54925E-11112.7856452
AAACACG34200.0111.3016365
CAGCGTT1600.099.694455
ACAGCGT1550.098.232664
TAGTAAC89200.097.296592
GTAGAAA41350.096.808941
CACAGCG1600.095.162893
AACACGT8700.094.173246
AGTAACA92800.093.600273
GGTGCGT700.093.2207953
ACGTGCA21000.091.4944842
Produced by FastQC (version 0.11.5)