FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_121_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_121_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727140
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT115730.4243639857139714No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC100950.370168014843389No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT92150.3378997777891858No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG50570.18543235770807512No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA49120.18011543228437119No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA48180.1766685978717631No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC40190.1473705053645944No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT38610.14157689007531699No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA30020.11007869049627081No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG28920.1060451608644954No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC28760.10545846564532807No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC27970.10256165800068937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC25800.0120.562521
GCACGCA26400.0117.5327152
CACGCAC28750.0108.682183
GTAACAA72650.0102.483464
CGCACTT33000.097.7608645
AAACACG24000.095.152085
ACGCACT34150.095.105634
GAAACAC25050.091.453074
TAACAAG85100.087.745865
TAGTAAC90100.083.198352
CGAGCTA2300.081.952925
AGTAACA98300.076.331853
GTCTCGC6650.075.2319261
GTAGTAA103250.074.226611
AACACGT6900.072.496826
ACGTGCA10350.071.446142
CTCGCTC7250.067.997023
GAAGCGG7750.067.360531
AACACGA29400.060.6606036
ACAACGC7650.058.7480558
Produced by FastQC (version 0.11.5)