FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_11_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_11_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547525
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT108340.7000856205877126No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC101130.6534950970097413No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA74960.48438635886334636No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA54460.3519167703268122No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC48250.3117881778969645No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT41850.27043181854897336No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT38330.24768582090757824No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG37310.24109465113649214No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG32160.2078157057236555No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA28600.18481123083633544No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT28490.18410041841004182No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA28090.1815156459507924No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA25740.1663301077527019No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA25580.16529619876900212No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT25330.1636807159819712No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATG24020.15521558617792927No Hit
GTTTATATGGTCTCCAGAGACAACGTTTCATGCTCCATCTGTCTATAAGG23030.14881827434128692No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT22660.14642735981648117No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC21710.14028852522576374No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA20310.13124182161839065No Hit
GTAAGAAGTACAGCAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA19900.12859242984765998No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT19890.12852781053617873No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT19760.12768775948692265No Hit
GCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG19670.12710618568359153No Hit
GAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTGTTAAAAC19550.12633075394581672No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT18950.12245359525694254No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT18930.12232435663398007No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG18820.12161354420768646No Hit
ATTGTATTCATTGAATTAATGGATAAATTTATATTAGCTCAAGGCCCACC17830.11521623237104409No Hit
CTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTGATGCTAGCTA16940.10946511364921406No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTGAT16630.10746191499329576No Hit
CTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAACA16030.10358475630442157No Hit
TTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATACTG15980.1032616597470154No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT15980.1032616597470154No Hit
AACACGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACA15880.10261546663220304No Hit
CCATATAAACAATAAAAATAGCTAGCATCAATGTTACAGCCAAACTAGAA15510.1002245521073973No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACG100.0070907436145.003361
GCGCGCA100.007092799144.98932
TCGTACG100.0070941695144.97993145
AGCACGC23850.0125.2439961
GCACGCA24400.0122.7061162
CACGCAC24500.0122.205273
GAAACAC14000.0111.331084
GTAACAA47800.0111.3202444
AAACACG14800.0105.313185
TAGTAAC54450.098.390352
ACGCACT32150.096.0582354
CGCACTT32500.095.692945
ACGTGCA7050.093.574662
GTAGTAA57850.093.368631
AGTAACA58050.092.538393
AACACGT3650.089.3769766
TAACAAG60400.088.2178345
AAGAGCC10700.079.264739
ACAACGC7500.077.322638
CAACGCA8200.073.373999
Produced by FastQC (version 0.11.5)