FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_117_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_117_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3303315
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT70330.21290733702356573No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC61820.18714533733537372No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC45400.13743769516379759No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT43510.1317161699686527No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA41700.12623682573414888No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT41340.12514701141126414No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC40570.12281601966509399No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA40400.12230138512373176No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT40200.12169593272212914No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA40100.12139320652132782No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA39800.12048502791892388No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC39490.1195465766964398No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC39450.11942548621611926No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG38810.11748803853099084No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT37330.11300769075913136No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA36400.11019233709167911No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT36150.10943552158967583No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT35990.10895115966839371No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG35800.10837597988687121No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA35320.10692289412302491No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG35270.10677153102262424No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG35150.10640825958166265No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT33990.1028966356523674No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTG100.0070902216145.00945
CGGGCCG100.007095037144.97647145
CGTACGT100.007095037144.97647145
GTAACAA46950.0103.931134
GAGCAGA5200.094.833941
GAAACAC16850.094.23464
AAACACG18400.087.084445
TAACAAG60500.080.6539845
AACACGT4050.080.5607766
ACGAGGA651.5679689E-978.0642559
ACACGTG4200.077.665977
TAGTAAC66600.074.7908862
AACACGA17500.073.333336
CAACGCA4800.064.9373869
TCACATG1150.063.047562
AGTAACA79650.062.8100973
CACGAGC19650.062.7124758
ACACGAG20900.061.3895657
ACGTGCA4800.060.420582
GAAGCGG9950.059.0364381
Produced by FastQC (version 0.11.5)