FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_110_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_110_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401610
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA27110.11288260791718888No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA24080.1002660715103618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC10100.067.4673848
AACACGA13700.065.626456
ACACGAG14550.062.2779587
AACACGT8400.062.1485526
TAGTAAC47500.061.0582242
AAACACG23500.060.782165
CAACGCA12700.059.3631559
GTAGTAA49150.059.310911
CACGAGC15700.058.177938
AACAACG12300.055.4000437
GAAGCGG15950.051.3747631
GTAACAA57850.049.8835684
AGTAACA60150.048.217223
TAACAAG62650.046.408885
ACGAGCT3400.044.7741939
ACGAGCA11900.043.8604289
ACACGTG12100.043.1354687
AGCGGAG18700.042.263233
GAAACAC33550.042.1424644
AAGCGGA19600.041.432372
Produced by FastQC (version 0.11.5)