FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_10_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_10_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177930
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT58810.18505756892064962No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA55150.17354063808831535No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT54100.17023660055444897No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC49910.1570519174431155No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA49520.15582470350196512No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT47380.1490907603377041No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA46870.147485942106969No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG43040.13543407186438972No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC40990.12898333191731726No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT39210.12338220162181042No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC38780.1220291195841318No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT37490.11796987347109597No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG36860.11598745095077613No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC36560.11504344022681431No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGCTCTTCCGTGACC36180.11384769330979601No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG35350.11123593030683496No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT35350.11123593030683496No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC35270.11098419411377848No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC34620.10893883754519451No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT34490.10852976623147773No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA34230.10771162360404414No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC34140.1074284203868556No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT34090.10727108526619529No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA34040.107113750145535No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG33140.10428171797364952No Hit
TCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT32290.10160702092242434No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA32040.10082034531912283No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG31970.1006000761501984No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT31870.1002854059088778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA45100.0105.7703864
GAAACAC12650.0103.729624
AAACACG13650.096.130385
TAACAAG53800.088.396745
ACACGAG13050.079.428947
GAGCAGA6050.079.100361
AACACGA13250.078.241086
AGCACGC1700.076.773881
CAACGCA5000.075.385289
AACACGT3750.073.462726
ACGTGCA5750.073.126522
ACGTAAA300.001937631572.48585145
TAGTAAC67500.071.851712
AGAAGCG4050.071.60115
GCACGCA1950.066.919492
GAAGCGG7550.066.2662051
ACACGTG4250.064.8108757
AGTAACA76050.064.0596853
CACGCAC2200.062.6102833
CACGAGC16250.061.1111458
Produced by FastQC (version 0.11.5)