FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_109_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_109_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146055
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC28300.081.4722062
GTAGTAA29900.079.304161
AGCACGC9800.063.634371
AAACACG22200.062.7071465
GCACGCA10300.060.5382732
AACACGA17250.058.844756
AGTAACA41700.054.9439433
ACACGAG19000.053.413647
ACGAGCG953.1452146E-853.413649
CACGAGC18800.052.8251088
CAACGCG701.698037E-551.7785269
ACAACGC10750.050.574378
TAACAAG45750.050.2385335
GTAACAA46850.048.7494544
ACGAGCA7700.048.0128149
GAAGCGG11450.045.5980641
GCCGAAC1002.7382303E-643.493968
AACAACG12550.043.320687
AAGCGGA12350.041.6831132
GAAACAC34300.041.4315074
Produced by FastQC (version 0.11.5)