FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_105_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_105_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2946228
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT100780.3420644973844522No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA85440.28999792276768804No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC68150.23131271578438597No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC64720.21967071116016818No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA57380.19475750009843093No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT54430.18474469728751475No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT53030.17999285866538503No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA51370.17435853572771695No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA51360.17432459402327316No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT50400.1710661903966699No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT50310.17076071505667587No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT49980.169640638810031No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG47860.16244499746794885No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATG46900.15918659384134562No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT44520.1511084681837251No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA42950.14577962058605104No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT42670.1448292528616251No Hit
ATCCAATGATGACCAATAACCCCATATACATCTTCGGAGCTTATGTGTAC42450.14408253536386187No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC41660.14140114071280294No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA39980.1356989343662473No Hit
ATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATA37580.1275529252997392No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC37240.12639890734865053No Hit
ATATAAATCCTTACTTTCTCTTCATAGATGTACCCATACAGGCAGCAATT37200.12626314053087542No Hit
CTTCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACA36620.12429452167313595No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT36490.12385327951536676No Hit
GTACAATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCA36150.12269926156427813No Hit
ATCTGGGACTCATAGCTGGAGAACTAAAAGGAACAGATCTATACTAAATA33710.11441748567999489No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACCAAT32720.1110572569400603No Hit
ATATACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCAT31640.10739155286013168No Hit
CCCATACAGGCAGCAATTTCAACAACATTCCCATACACTGGTGTTCCCCC30220.10257183082911438No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC30140.10230029719356412No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA29960.101689346513576No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCC100.0070945714144.9794145
AGCACGC7350.0106.54261
GCACGCA7450.0105.0964362
CACGCAC7950.098.48663
GAAACAC21150.098.3766564
AAACACG22550.092.590515
ACTCGAC259.390077E-486.99653
AAGAGCC18500.082.677449
GTAACAA64550.080.7519764
AACACGA20550.079.3763666
GAGCAGA8650.077.956741
CAACGCA7400.075.4284749
ACAACGC7450.074.922248
ACACGAG21800.074.817357
CTCGACC300.00193642772.497084
TCGACCT300.00193642772.497085
CGTCAGA300.00193642772.497082
TAACAAG72750.072.148295
ACACGTG6450.070.8038947
AACACGT6600.069.201766
Produced by FastQC (version 0.11.5)