FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_102_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_102_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188003
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC7550.087.41121
TAGTAAC38100.076.886422
CGGAGCG300.001935073972.5092245
GTAGTAA41050.071.9041441
AAACACG23950.070.8440865
GCACGCA9650.068.376572
GTAACAA43500.067.175224
AACACGA19250.061.774096
AGTAACA49800.058.8227463
ACACGAG20400.058.279747
AACACGT8950.056.7111246
CACGAGC21150.054.8420338
TAACAAG54100.054.549455
ACGAGCC7000.053.8529179
CGCACTG2850.053.427855
CACGCAC12500.053.366793
GAAACAC35650.047.593714
ACGCACT17000.047.3442574
CGCACTT12100.047.3407365
ACGAGCG951.9229774E-645.7858779
Produced by FastQC (version 0.11.5)