FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_100_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_100_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450364
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC87730.3580284398562826No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT86430.35272310562838827No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT69530.2837537606657623No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT53180.21702898018416855No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC49730.2029494393486029No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT42190.17217850082681593No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC38420.15679303156592245No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA33650.13732653597587952No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC32460.13247011464419162No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT30980.1264301956770504No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC30890.12606290330742698No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC30810.12573642120109502No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC30740.12545074935805456No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT30650.12508345698843112No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC30590.12483859540868214No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG30280.1235734772466458No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC28400.1159011477478448No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG28380.11581952722126182No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA28270.11537061432505538No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG28080.11459521932251698No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT27770.11333010116048065No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA27700.11304442931744019No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA27670.1129219985275657No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA27370.11169769062882086No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT27220.11108553667944843No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG26730.10908583377816519No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT26410.10777990535283738No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA25480.10398455086672838No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA25390.10361725849710492No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA25270.10312753533760699No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT25230.10296429428444101No Hit
GTCTACACCTGTGACAGTGTCCCACAGCAGCTGTCCTGACCCCATTAAAC25160.10267862244140054No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA25090.10239295059836008No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA24820.10129107348948972No Hit
GAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAA24620.10047486822365984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA48100.0117.871154
GAAACAC19700.0112.616244
AAACACG19850.0112.1304865
AGCACGC6850.0110.0974351
CACGCAC6900.0109.277333
GCACGCA7050.0106.952282
AACACGT5650.0100.09036
TAACAAG58000.097.751765
CGAAGTT259.3877135E-487.0015643
GAGCAGA5150.084.484781
ACACGTG6700.084.390737
TAGTAAC72550.079.646512
TAGAAAC30250.078.3733142
AACACGA21150.078.157436
CGCACTT9800.076.200355
ACGCACT10100.076.0904854
AGTAACA78450.073.748943
ACGTGCA9900.072.501312
CGGAGCA13900.071.458122
GAAGCGG6850.070.9281541
Produced by FastQC (version 0.11.5)