FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11045185
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG123830.11211220092737241No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG120810.10937797782472633No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA25950.0101.667671
GAGCAAA215550.069.402991
GCAAAAG216400.068.818633
AGCAAAA233700.063.8170662
GCAGGGT114900.059.0111859
CAAAAGC292000.051.592184
AGCAGGG200850.048.3881848
AAGCAGG371200.047.4635287
CGAAAGC63950.047.3167653
AAAGCAG412500.042.9507186
GGATAGC20500.042.774355
GCAGGTA109050.040.8797959
TCGTACT10500.039.7023432
AAAAGCA382950.039.4331445
CGCGTAA2800.036.344883145
ACTCCGA3250.033.1789051
GGTAGTT15950.032.4486357
AGCGAAA98150.031.4210821
AGCGCCC4400.031.388763145
TCTCGGG25350.031.255167145
Produced by FastQC (version 0.11.5)