FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_PLASMID_CONTROL_P1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_PLASMID_CONTROL_P1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2909102
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT111800.3843110348141798No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG83290.28630828344966935No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT82920.2850364132986743No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG66450.2284210041449217No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT63310.21762729529593666No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT63270.21748979582015346No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT61710.21212731626460676No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA59400.20418672153812412No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT54430.18710241167205552No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA53810.18497116979741515No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA49540.17029310075755338No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG46600.1601868892874846No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC44930.15444628617353398No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG41580.1429307050766869No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA41260.1418307092704209No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG39330.13519635956387915No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT38570.13258386952399745No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC38020.13069325173197777No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA37640.1293870067120369No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT37350.12839013551260836No Hit
AACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAG36290.12474639940435227No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTTT34970.12020891670350506No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG34870.11986516801404695No Hit
ACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGATCATT33470.11505268636163325No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG33370.11470893767217512No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT33170.11402144029325889No Hit
CTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGC32220.11075582774340673No Hit
ATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGC32090.11030895444711117No Hit
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT31750.10914020890295355No Hit
TCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT31540.10841833665509151No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT31060.10676834294569251No Hit
CTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCT30830.10597772095993885No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT30750.10570272200837234No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT30690.10549647279469747No Hit
ATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT30640.1053245984499684No Hit
TCTCTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCA30030.10322773144427386No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT29660.10195586129327881No Hit
CCTATATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGG29450.10123398904541675No Hit
TTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCC29260.10058086653544633No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCCC3700.076.43964145
TCTGACC2600.064.152145
GAGTGGC2150.064.035346
AGAGTGG2250.061.190375
CGTCAAA1600.054.389732145
CTTCTAA25950.052.5130121
CCAGAGT2700.050.9919743
AGTGGCC2850.050.849857
GTCGGCC2150.050.5951145
TAGATGG26800.049.7536438
TTCTAAT28050.048.571532
TACTGAT14450.045.131447
TATGTTT2100.044.893116145
CGCTGGG2350.043.203197145
CGACGAA8750.042.268593145
CATATAC15100.042.2296753
ACTGATG15150.042.0931938
GCGATTT1903.6379788E-1241.985058145
TCTGTCC1755.2750693E-1141.439796145
ATCTTAG700.001058070841.4198381
Produced by FastQC (version 0.11.5)