FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_NEGATIVE_CONTROL_P1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_NEGATIVE_CONTROL_P1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG40.3913894324853229No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA40.3913894324853229No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG30.29354207436399216No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG30.29354207436399216No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT30.29354207436399216No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA30.29354207436399216No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30.29354207436399216No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA30.29354207436399216No Hit
TAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTA20.19569471624266144No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA20.19569471624266144No Hit
ATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATA20.19569471624266144No Hit
CGTTTGATCATTCTGATTAACTCCATTGCTATTGTTCCAACTCCTTTCAC20.19569471624266144No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC20.19569471624266144No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC20.19569471624266144No Hit
TATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTG20.19569471624266144No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT20.19569471624266144No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC20.19569471624266144No Hit
TATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTGACA20.19569471624266144No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT20.19569471624266144No Hit
GTCTGTCTCTCTCCCCAGCTCTGTGTCAAATAAATAAATGACATCTTTAA20.19569471624266144No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA20.19569471624266144No Hit
TGTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGTCATTCTAAGT20.19569471624266144No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG20.19569471624266144No Hit
TTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCA20.19569471624266144No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT20.19569471624266144No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG20.19569471624266144No Hit
CTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAATT20.19569471624266144No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC20.19569471624266144No Hit
GAAGTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTC20.19569471624266144No Hit
ATGCTTAATGCTGGGCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACA20.19569471624266144No Hit
TGCTTAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAA20.19569471624266144No Hit
AGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAA20.19569471624266144No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA20.19569471624266144No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT20.19569471624266144No Hit
GTTAGTAATCTCGTCAATGGCATTCTGTGTGCTCTTCAGGTCGGCTGCAT20.19569471624266144No Hit
CTTGTATAGTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCAT20.19569471624266144No Hit
GTTCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTG20.19569471624266144No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC20.19569471624266144No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT20.19569471624266144No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT20.19569471624266144No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATATCGTTTGTTTTGCTC20.19569471624266144No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA20.19569471624266144No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTT20.19569471624266144No Hit
ATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATG20.19569471624266144No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT20.19569471624266144No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG20.19569471624266144No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG20.19569471624266144No Hit
CCCTTATGACAAACACATCCCCCTTGGAACCGATTCTTACACTGTTGTCT20.19569471624266144No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC20.19569471624266144No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA20.19569471624266144No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)