FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_DEMUXING_CONTROL_P1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_DEMUXING_CONTROL_P1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT100.3933910306845004No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA70.2753737214791503No Hit
GGTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGA60.23603461841070023No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT60.23603461841070023No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG50.1966955153422502No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA50.1966955153422502No Hit
GGTCCATTCTGATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGC50.1966955153422502No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT50.1966955153422502No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG50.1966955153422502No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA50.1966955153422502No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT50.1966955153422502No Hit
GTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTC40.15735641227380015No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT40.15735641227380015No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT40.15735641227380015No Hit
GCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATTTCACCAT40.15735641227380015No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG40.15735641227380015No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG40.15735641227380015No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT30.11801730920535011No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT30.11801730920535011No Hit
GATTGCTGACTCCCAGCATCGGTCTCATAGGCAAATGGTGACAACAACCA30.11801730920535011No Hit
GGCCTCTGCTGCTTGCTCACTCGATCCAGCCATTTGCTCCATAGCCTTAG30.11801730920535011No Hit
CTCAATATGAGTGCAGACCGTGCTAGAAAAGTGAGATCTTCGAACTCAGC30.11801730920535011No Hit
CTTTGTATCAGAATGGACCAGGCGATCATGGATAAGAACATCATACTGAA30.11801730920535011No Hit
TCTCTGTAGTGACTTCAAATTATAACAGGAGATTTACCCCGGAAATAGCA30.11801730920535011No Hit
GAGCTCAATTGCTCCCTCAGCTCCTCATAGTCGATGAAATCTCCTGGATA30.11801730920535011No Hit
GACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCA30.11801730920535011No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA30.11801730920535011No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC30.11801730920535011No Hit
ATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTGAG30.11801730920535011No Hit
GACCAGTCCCTTGACATTTCCTCAAGAGTCATGTCAGTTAGGTAACGCGA30.11801730920535011No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA30.11801730920535011No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA30.11801730920535011No Hit
ATATACACCCAGTCACAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCC30.11801730920535011No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC30.11801730920535011No Hit
ATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGTTCCTT30.11801730920535011No Hit
TGATGACACTGAGCTCAATTGCTCCCTCAGCTCCTCATAGTCGATGAAAT30.11801730920535011No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA30.11801730920535011No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA30.11801730920535011No Hit
AGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACTG30.11801730920535011No Hit
CTGTACCTGCGTCGCGTTACCTAACTGACATGACTCTTGAGGAAATGTCA30.11801730920535011No Hit
AATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATTT30.11801730920535011No Hit
GTGTTCTTCCCTGCAAAGACATCTTCAAGTCTCTGTGCGATCTCGGCTTT30.11801730920535011No Hit
CCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGC30.11801730920535011No Hit
GTCCAAAATGCCCTTAATGGGAACGGGGATCCAAATAACATGGACAAAGC30.11801730920535011No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC30.11801730920535011No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGG100.0066762073144.999984
AAAAAAA1008.336428E-921.75145
Produced by FastQC (version 0.11.5)