FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_CW_B4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_CW_B4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5103622
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG225830.44248966714227655No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA135460.26541934336046047No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG116930.22911179550523136No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT105860.20742131764460614No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT93000.18222352674238024No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA91450.17918646796334056No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC87620.17168199368997156No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT78640.15408664669914818No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT77490.15183334502437681No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA76960.1507948668612213No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG74700.1463666392221054No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT72370.14180125408974253No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT69910.1369811478984925No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA67260.13178875708271498No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT67030.1313380967477607No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT64950.12726255980556556No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT64480.12634164520805027No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA61770.12103169082663254No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG61760.12101209689902584No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA60880.11928783126963556No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA57950.11354681048087024No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC56780.11125432095088546No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA53220.10427888272289759No Hit
GAGTAGAGGCTTTGGGTCCGGCATCATCACCTCAAACGCATCAATGCATG52640.10314243492170853No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA52500.10286811993521464No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT52020.10192761141009268No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT51990.10186882962727256No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA51410.10073238182608353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA15900.0129.48271
GCAAAAG121100.0103.895863
ACGGCCC1000.094.2702145
GCAGGGG38050.088.0011759
AGCAGGC9250.086.971738
TGACGCC6050.082.703674145
AGCGAAA36850.082.426541
GCAGGGT78400.081.1670159
ATTAGTA3350.077.901931
AGCAAAA164150.076.826982
AGCAGGG129250.075.476448
GTTGACC1800.072.51554145
CCTGTTT1900.072.51554145
CCTCGCC1450.070.015145
AAGCAGG211850.069.240777
CAAAAGC184900.068.320824
GTCGCCC5100.066.82805145
CGACGAA9350.063.59652145
GAGCAAA202650.063.066141
TATTAGT1750.062.136071
Produced by FastQC (version 0.11.5)