FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_740-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_740-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187036
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG14410.12139480184257259No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC13920.11726687311926513No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC12420.10463035661934432No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12380.1042933828460131No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCC3550.073.52826145
GAGCGAA6100.062.9872971
GCGAAAG6700.056.257523
AGCGAAA6800.054.364242
TACGTGT1900.053.410483
CGAAAGC7350.050.296174
GCGATTA1301.3096724E-1050.197178145
GAGCAAA33550.048.617981
GCCGACC2550.048.338024145
GTCGCCC2500.046.404507145
GTTACGT2250.045.1078871
GCGACTA1401.4173565E-841.4203724
TTACGTG2450.041.4203722
ACTCCGA2650.041.03481
CAAAAGC41650.039.5059854
ACGTGTT2600.039.0307354
GCAAAAG43050.038.5579873
CGTATCC3250.037.926758145
AAAGCAG54050.037.1480566
TCTCGGG4550.036.65191145
Produced by FastQC (version 0.11.5)