FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_644-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_644-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140183
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAA2950.078.655876145
GAGCAAA30850.066.969731
GAGCGAA7850.062.7954371
GACGAAA5400.061.768536145
AGCGAAA11100.059.430211
GCGAAAG12100.055.7046052
GCAAAAG39050.051.77723
CAAAAGC38950.051.5402834
CGAAAGC13550.050.808893
TGACGCC3350.047.61909145
AGCAAAA43600.046.211692
AAAGCAG63150.039.824446
AAAAGCA57550.035.136015
GCCGACC1851.6418016E-731.356058145
GTATAAG3750.028.9967171
AAGCAGG85750.027.4689967
TCTCGGG10100.022.255817145
CGTATCC5001.8189894E-1221.753267145
GTCGCCC3106.2365325E-721.05155145
GCAGGGG31750.021.0008879
Produced by FastQC (version 0.11.5)