FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_644-2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_644-2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCTCGTCA30.2918287937743191No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC30.2918287937743191No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT30.2918287937743191No Hit
CAATATATTGTTTCTCCAACTCTTGATAGTGTCTGTTATTATGCCGTTGT30.2918287937743191No Hit
CTGCGCCTCTCTGGATTTCTCATTTCCTGGCCCCAAAGGGAACACTTAGG20.19455252918287938No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG20.19455252918287938No Hit
GATCAAAAGTCCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGATATCGA20.19455252918287938No Hit
CTTCTAGGTATTTATTTCTTCTCTCATCAAAAGCAGAAAGCACCATCCTC20.19455252918287938No Hit
ATGTTGACAAAATGACCATCGTCAACATAGAGCCTGTCTCTTATACACAT20.19455252918287938No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC20.19455252918287938No Hit
TTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTT20.19455252918287938No Hit
CAGCGAGACACTTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACC20.19455252918287938No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA20.19455252918287938No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG20.19455252918287938No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA20.19455252918287938No Hit
GTAAATAAACACAATAAATTCTTCTCCAATGACCATATCCTGACTCCAAT20.19455252918287938No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC20.19455252918287938No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC20.19455252918287938No Hit
TTCAAGATCCATTCCACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGAT20.19455252918287938No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG20.19455252918287938No Hit
ACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGA20.19455252918287938No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT20.19455252918287938No Hit
CATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCA20.19455252918287938No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG20.19455252918287938No Hit
ATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATT20.19455252918287938No Hit
CTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAAATTCCAATTGTTA20.19455252918287938No Hit
GTATTTATCGTCGCTTTAAATACGGTTTGAAAAGAGGGCCTTCTACGGAA20.19455252918287938No Hit
GTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA20.19455252918287938No Hit
GTCTTTAGCCATTCCATGAGAGCCTCAAGATCTGTGTTCTTTCCTGCAAA20.19455252918287938No Hit
ATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGT20.19455252918287938No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)