FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_562-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_562-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823486
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG38480.21102437858036752No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA28080.15399076274783574No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC21240.11648019233490138No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT18780.10298954858989869No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4700.0101.7962341
TGACGCC4200.089.77079145
GCAGGTA11150.063.0554969
TCGCGCC350.00356557562.14901145
AGCGAAA9250.058.77671
GTTACGT1500.057.993011
GAGCAAA53850.053.577571
CGAAAGC10450.053.401423
GCGAAAG10500.052.456912
TCCGAGG3400.051.1576653
ACTCCGA3250.049.0710071
TACGTGT1800.048.315573
AAGCAGG73400.046.1104357
GCAAAAG63850.044.7212263
TTACGCC657.3346E-444.6198145
CTCCGAG4500.043.4840132
GACGAAA12050.043.32379145
AGCAAAA67350.042.5047872
CAAAAGC70800.041.457224
GTTATGT4700.040.1015471
Produced by FastQC (version 0.11.5)