FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_559-3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_559-3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505870
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAA3850.077.22363145
GACGAAA7150.064.908424145
GAGCAAA42550.055.202291
TGACGCC3350.054.115578145
GAGCGAA12000.051.3510481
GCGAAAG11450.051.2731672
CGAAAGC12500.046.966223
CAAAAGC50000.046.8212664
AGCGAAA12800.045.876121
GCAAAAG50650.045.648013
ACTCCGA2900.044.997271
AGCAAAA57050.040.78122
GCCGACC1859.640644E-1139.19723145
CGTCGAG750.00148576538.6553272
AAAGCAG76250.038.3992966
GTCGCCC3150.032.228836145
AGTCGAA1902.0831249E-730.5173632
GTTACGT2151.8128048E-830.3469941
AAGCAGG88750.030.132827
AAAAGCA79750.029.173265
Produced by FastQC (version 0.11.5)