FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_545-3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_545-3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116447
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA9550.081.973311
GCGAAAG10250.077.071952
CGAAAGC10900.073.140833
GAGCGAA3700.066.6084441
GACGAAA4200.062.155632145
GAGCAAA22550.043.3949621
CAAAAGC25500.038.3696024
TACGAAC1001.393907E-436.2395829
TATACGA1001.3942744E-436.237967
GCAAAAG27400.034.6508983
TGACGCC3350.032.46936145
AGCAAAA30800.030.8257942
GAAAGCA31700.026.2925224
CGGATCC2001.0639145E-525.380219145
AAAAGCA42800.023.3684025
AAAGCAG54650.022.4124976
ATACGAA2053.6739322E-421.2134158
TCGCCGG5600.020.718544145
GGAGCGA1850.005053011219.590721
CCTTCGC2307.9781364E-418.9076069
Produced by FastQC (version 0.11.5)