FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_540-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_540-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1679176
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC34990.20837601299685082No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC28560.1700834218688214No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA28150.16764174809549445No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG26790.1595425375303125No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA25810.15370634168187253No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA25230.15025226658789786No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG21800.12982558111835804No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA19330.11511598545953493No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG18350.10927978961109497No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC18000.10719543395093785No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT17700.10540884338508887No Hit
ACATTCTACTGAACAACCAATTGGGTTCATGGGCAAACCAAAATAGGAAC16930.1008232609327432No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA16850.10034683678185015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6100.0101.022781
AGCGAAA8550.073.750832
GTTACGT2950.071.269911
TGACGCC3150.069.05873145
CGAAAGC9250.068.9511954
GCGAAAG9900.066.620373
TTACGTG3250.062.4436762
TACGTGT3350.060.5778853
TTCGCAG1450.059.9810523
GTCACGT2600.055.768231
GAGCAAA46000.054.058821
TCGCAGA1650.052.7106254
GCGATTA2350.052.45525145
AATGGGT11500.046.6360557
TTTCGCA1900.045.776382
ACTCCGA5100.045.4893841
CTCGACT2400.045.2995458
CATTCTA23700.044.6496732
GACGGCT2150.043.823373
CTACTGA27600.043.0648236
Produced by FastQC (version 0.11.5)