FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_531-1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_531-1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623325
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG74030.45603930204980514No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA58330.359324226510403No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA32230.19854311367101474No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC31030.19115087859793942No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT30520.1880091786918824No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT30220.18616111992361356No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT30140.1856683042520752No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA27160.1673109204872715No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG27010.1663868911031371No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC24720.15228004250535165No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC24210.14913834259929468No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT23610.145442225062757No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT22750.14014445659371969No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC22110.13620193122141283No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA21750.13398426069949024No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG21380.13170498821862536No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT20570.1267152295442995No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA20430.1258528021191074No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC20320.12517518057074214No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC19850.12227988850045432No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC19240.11852216900497436No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG18950.11673571219564782No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT18450.1136556142485331No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG18430.11353241033064852No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT18310.11279318682334098No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT18000.11088352609612986No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT17870.11008270062988001No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT17690.10897386536891872No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC17490.10774182619007285No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC17220.1060785732986309No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC17170.10577056350391943No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC17150.10564735958603483No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA17040.1049697380376696No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT16970.10453852432507353No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT16890.10404570865353518No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT16490.10158163029584341No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4250.0134.741121
CCTTACC1800.0100.71408145
TCACGCC301.5982125E-596.685524145
GCAGGTA11200.087.3542569
CGCGAAA259.3805365E-487.01697145
AGGCTCC4050.082.36173145
GCAGGGT24650.079.968759
CCTCGCC1000.079.76556145
TGACGCC2500.078.31527145
AGCGAAA9000.072.487311
GCGTCAG300.001937304572.487311
TAGCGCT300.001938963472.471684
TCTGACC1750.070.44231145
CTTACGG651.396711E-766.93613145
GCGAAAG9850.066.219822
CGAAAGC10450.062.4157943
AGCGCTA350.003572428662.1185845
CTAGCGC350.003572428662.1185843
GTAGAAA11450.062.0415421
AAGCAGG65400.061.608217
Produced by FastQC (version 0.11.5)