FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_528-3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_528-3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387638
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8450.075.488671
GAGCAAA42650.063.2236671
AGCGAAA13900.059.970661
GCGAAAG14050.059.8355332
CAAAAGC47400.056.8736084
CGAAAGC14950.055.7425963
GCAAAAG51600.051.540293
AGCAAAA55550.047.4891972
GTTACGT2400.045.3039251
AAAGCAG75950.044.3681536
GTCGCCC3600.044.31475145
TGACGCC3000.041.091858145
TCGCCGC2800.036.257523145
AAAAGCA75500.035.898055
AAGCAGG91850.034.8729977
CGTGTAA6600.034.060093145
GCCGACC2900.032.506744145
TCCGGTA1000.00608623728.9892832
AGACGAC2603.741661E-927.8712943
TCTCGGG7300.026.820633145
Produced by FastQC (version 0.11.5)