FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_428-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_428-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886335
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG74970.39743735868761376No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA60740.32200006891670885No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC28650.15188182374816775No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT27700.1468456027163786No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT27680.14673957701044618No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC27300.1447250885977305No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA26780.14196842024348802No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA23490.1245271916176077No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT22650.12007411196844676No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG21690.1149848780836914No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA21510.11403064673029975No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT20940.11100891411122626No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT19850.10523051313791028No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC19250.1020497419599382No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC18900.10019429210612113No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6150.0113.148541
TGACGCC3550.083.74485145
GCAGGTA15550.075.9697959
AGCGAAA12600.070.7599261
CGAATAA7850.066.506714145
CGAAAGC13600.066.082973
GAGCAAA60950.066.004281
GTTACGT554.0677314E-665.8961641
GCGAAAG13600.065.550042
CCTCGCC1450.065.009674145
AGTCGGC606.8212066E-660.396815
AAGCAGG78300.059.6084487
CGACGGG400.006059588454.357135
GACGAAA13550.052.97836145
AGCAAAA74650.052.9143452
CTCGCCC1004.7008143E-850.75755145
GTAGAAA14250.049.849871
GCAAAAG82750.047.5596433
TTACGTG803.742594E-545.2988052
CGATGTT657.3501334E-444.600725
Produced by FastQC (version 0.11.5)