FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_427-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_427-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546500
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG27980.18092466860653086No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT27810.1798254122211445No Hit
GATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCACTACCTCCA26570.1718073068218558No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT25750.16650501131587456No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC22690.14671839637892015No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT22520.1456191399935338No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA21150.1367604267701261No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA21130.1366311024894924No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC19770.12783705140640156No Hit
GAAAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAATGGGAG19570.12654380860006464No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT18770.1213708373747171No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG17300.11186550274814096No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT16740.10824442289039767No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC16280.10526996443582283No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG16130.10430003233107016No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT15810.10223084384093115No Hit
CTTGAATGGAATGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATT15480.10009699321047527No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA11100.094.700991
CGAAAGC13000.081.39143
GCGAAAG13500.078.381972
GAGCGAA3550.069.432061
TCGACGG350.00356551262.148838145
TGACGCC3600.056.394314145
TATTACG400.006059280654.357362
TCTACAC6850.049.7251743
CAATTCG3100.046.759029
ATATGCG3150.041.4124535
CTACACT9400.038.5488244
CCGACGC1553.4695404E-837.422955145
ATCCCGA3900.037.1650246
CGTAACC2002.237357E-1036.253487145
GCACCGT5000.036.2358936
TATGCGT3800.034.3287436
GAAAGCA31250.034.322644
ATGCGTC3950.033.025127
CACCGTC4300.032.022427
AGCACCG5850.030.9708525
Produced by FastQC (version 0.11.5)