FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_286-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_286-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211833
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA17500.14440933693008856No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG16540.13648745330420942No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC16080.13269155073347566No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC15620.12889564816274188No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC15150.12501722597090523No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA13970.11527991068076213No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12420.1024893694098114No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT12270.1012515750932678No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5100.075.335561
TACGTGT2250.067.6347353
TGACGCC2650.065.67276145
GTTACGT2550.062.5427321
TTACGTG2500.060.873772
GGTTATA2750.060.6302721
GTCGTCT1206.002665E-1154.385254145
CGAAAGC7350.051.268314
GCGAAAG7600.050.5353473
AGCGAAA7500.050.2450182
ACGTGTT3200.049.8202254
GAGCAAA33400.049.0519521
GTATACG604.947949E-448.328481
GTCGAAT604.9560145E-448.3125152
ACTCCGA3300.046.1317251
GCCGACC2100.044.889416145
AGACGAC2500.043.479473
ATGGGTG8300.042.784548
AATGGGT8350.042.5283437
TCGCCGG4400.041.20095145