FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_283-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_283-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400630
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG36380.2597402597402597No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA24560.17534966407973554No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC18050.12887057966772095No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA17050.12173093536480012No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG16830.12016021361815755No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC15930.1137345337455288No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC15130.10802281830319213No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA15020.10723745742987084No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA14380.1026680850760015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4350.0113.29381
TGACGCC2800.082.88153145
AGCGAAA7800.063.1785622
GTTACGT3350.060.575881
CGAAAGC8100.059.9417954
GCGAAAG8350.059.8828663
ACTCCGA3000.055.5639421
GCAGGTA7450.055.4484679
GAGCAAA44850.052.194721
GTCGTCT1004.694266E-850.76494145
ATGTCGA1004.7180947E-850.7322961
CTCCGAG3450.050.413592
TCCGAGG3600.048.31133
AGTCGGC1056.9339876E-848.31135
AAGCAGG62450.045.8357777
GCGACTA1602.0008883E-1145.291844
GCGATTA1505.347829E-1043.512802145
GTTATGT7150.042.572561
GATATAC2750.042.1625863
AGACGAC2300.040.9595763