FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_283-3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_283-3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3206305
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG106470.3320644792058148No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA85780.2675353717129219No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA74280.23166854057864117No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT65750.20506470844164856No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC58700.18307678152889384No Hit
ATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATG54240.16916668875855542No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG54140.16885480327043123No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG52780.16461316063194237No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA50040.15606749825733982No Hit
GGATACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGG47390.14780253282204905No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA45090.1406291665951929No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC40580.12656313108079237No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA36280.11315205509145262No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT35290.11006438875902323No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT34520.10766287050046705No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG34470.10750692775640495No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA32700.10198655461660697No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT32490.1013315950915462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA11500.0131.745221
TGACGCC5100.079.62325145
AGCGAAA23450.070.1729051
CGAAAGC24850.066.491493
GCGAAAG25150.065.411232
GCAGGTA25450.064.639169
GAGCAAA118100.064.1435241
AAGCAGG144800.063.2590457
GTTACGT2250.061.2149541
AGCAGGT60400.056.0321928
AGCAAAA150500.050.272262
AAAGCAG182950.050.2262766
GTAGAAA27300.049.920811
CAAAAGC155100.049.2944834
GACAGTA5800.048.7282377
GTTATGT8550.048.3275951
CAATTCG19400.047.815199
GCAAAAG164650.046.171233
GAAGGCG3000.045.8976064
GTCGAAG2450.044.3693243
Produced by FastQC (version 0.11.5)