FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_199-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_199-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077984
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA16370.1518575414848458No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA13500.12523376970344643No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA12800.11874016682993438No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC12780.11855463531926262No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG12530.11623549143586548No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC12180.11298868999910945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2700.0110.077751
CGATTAA259.3798566E-487.01655145
GTTACGT1600.067.9595951
TGACGCC2400.060.428158145
AGCGAAA5000.059.4254382
TTACGTG1850.058.75952
TACGTGT1850.058.7567753
ACTCCGA3000.057.9921841
AGTCGGC1500.057.976045
TCTCGGG3400.057.58448145
GTCGCCC2050.056.596127145
AGTCCGC651.0924699E-555.77984145
CGAAAGC5200.055.7436074
GCGAAAG6050.052.703043
TCCGAGG3450.050.4116063
AACGGGT3250.049.0543753
GGTTATA1800.048.326821
TTTCGCA1351.9099389E-1048.3133662
GTAATGC1700.046.9054451
GAGCAAA27850.046.8518561
Produced by FastQC (version 0.11.5)