FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_197-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_197-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482740
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG28180.19005354950969153No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA19680.13272724820265186No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC16930.114180503662139No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG16900.11397817553987888No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA16240.1095269568501558No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4300.0111.262211
TGACGCC3100.074.84757145
GTTACGT2000.068.864561
TTACGTG2050.067.175872
TACGTGT2050.067.173613
GCAGGTA9900.060.0333029
AGCGAAA8100.059.951962
AGTCGGC1600.058.8893745
CGAAAGC8150.057.803573
GAGCAAA41500.052.5763661
GCGAAAG9550.052.365433
TCGCCGG6350.047.95844145
AGCAGGT16900.046.746968
GTAACTC2050.045.9608883
GGTTATA1900.045.7825361
GTCGTCT803.7329177E-545.317867145
AAGCAGG59700.044.6773157
TCTCGGG4350.043.338467145
AGACGAC2050.042.4254343
GACGCTC1053.822257E-641.433483145
Produced by FastQC (version 0.11.5)