FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_197-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_197-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547742
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG84050.5430491645248369No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA57380.37073362356258344No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA41010.26496664172710954No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30570.19751353907821848No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC29240.18892037561815858No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT27210.17580449454754088No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT25670.16585451580431362No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA23550.15215714246948134No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA23290.15047727592841703No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT23040.14886201963893209No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG22710.14672988133681195No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT22300.14408086102205664No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC22260.14382242001573906No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT22220.14356397900942147No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG21810.14091495869466616No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT21610.13962275366307822No Hit
ATATTCAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGA21430.13845976913464905No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC20840.1346477642914646No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC20800.13438932328514702No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA20640.13335555925987663No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA20580.13296789775040027No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA20010.1292851134103746No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT19890.12850979039142182No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC19800.12792829812720727No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG19740.12754063661773088No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT19290.12463317529665797No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTCTGGAATCATGT18920.12224259598822027No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG18830.12166110372400568No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC18140.11720299636502725No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC16800.10854522265338798No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT16260.10505626906810049No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT16130.10421633579756832No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG15960.10311796152071856No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC15920.10285952051440099No Hit
GAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC15900.10273030001124218No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG15830.10227802825018639No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT15760.10182575648913061No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGG100.0070880437145.02182145
GAGCGAA6700.0128.750561
GAGCAAA56900.083.06391
GCAGGTA19400.079.576479
TGACGCC2650.076.6153145
AGCGAAA14100.076.602721
CGGCGGG300.001934787572.51092145
GCGAAAG15100.071.99582
AAGCAGG78700.071.739037
CGAAAGC15750.068.564363
CTCCGAG754.8694346E-967.644042
GTAGAAA11450.063.3098871
GACAGTA4150.062.8705377
GCAGGGT32850.061.9980139
CACCTAC6800.060.751743
AGCAGGT38750.059.291768
AGCAAAA80850.058.446752
ACTCCGA753.7662176E-757.991851
TCCGAGG753.7712562E-757.980613
AAAGCAG102250.055.2871326
Produced by FastQC (version 0.11.5)