FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_156-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_156-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785590
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG30130.16873974428620234No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC26480.1482983215631808No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC26340.1475142669929827No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG23530.13177717169114972No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA22460.12578475461892147No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA22430.1256167429253076No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA21790.12203249346154493No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT20590.1153120257169899No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA19920.11155976455961335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3200.088.33691
ACGGTAT350.003573333162.114876
GTTACGT4000.059.7972871
GCCGACC3050.059.442764145
ATACCGG753.7746213E-757.973886
TACCGGA753.7746213E-757.973887
GATACCG805.894635E-754.3505135
AGCGAAA5450.053.197481
CGATACC553.2268555E-452.7035264
ACTCCGA6050.051.5158231
TGACGCC3400.051.190712145
CGAAAGC5950.049.9354823
GTCGCCC3650.047.684498145
GATATAC5950.047.4996033
CGATTAA1551.4551915E-1146.78721145
GCGAAAG6700.045.4285622
GTCACGT1950.044.604041
TCCGAGG7550.043.192463
CTCCGAG8450.039.4507832
GTAATGC2400.039.2608451
Produced by FastQC (version 0.11.5)