FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_123-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_123-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666345
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC38300.14364232685567696No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA35890.13460373657572444No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA33650.1262027232034864No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG33620.1260902096315368No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC32900.12338988390474602No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC29770.1116509678980027No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA29450.11045082313054012No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA28280.10606279382450509No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8100.0106.5041661
TGACGCC6250.073.088104145
GTTACGT5450.063.8483121
CGAAAGC13850.062.2736174
TACGTGT5600.062.12413
AGCGAAA13900.062.0519372
TTACGTG5950.058.4719352
GCGAAAG15850.057.1593973
ACTCCGA8100.051.01461
AGTCGGC2900.049.984915
GGTTATA5600.047.898111
GAGCAAA76600.045.5219651
TCTCGGG8300.045.426723145
GGTCGTT2600.044.6027537
TCCGAGG9150.043.5660823
GTCGTCT3500.043.504826145
TCGCCGC2900.042.504715145
ACGTGTT8250.042.1690834
GATAGCC4200.039.706783145
AAGCAGG108650.039.625237