FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n02_TR_123-1_v2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n02_TR_123-1_v2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2058480
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG86330.4193871205938362No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA64460.31314367883098204No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT30260.14700167113598384No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30200.14671019392950138No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT29290.14228945629785084No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA28550.13869457075123393No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA26930.13082468617620768No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC26510.12878434573083053No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG26460.1285414480587618No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC26290.1277155959737282No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT24990.12140025649994171No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG24740.12018576813959815No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT23010.11178150868602074No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT22320.10842952081147254No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA21940.10658349850375035No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT20800.10104543158058372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA607.2759576E-1296.68209145
GAGCGAA10750.087.664961
TGACGCC3800.076.32797145
GCAGGTA18300.075.250489
GAGCAAA72800.068.4095151
AGCGAAA17450.066.0530551
CGAAAGC17750.065.735893
GCGAAAG17700.065.513732
TCGCGCC350.00356478562.152775145
AGCAAAA84050.058.8074462
AAGCAGG106750.054.2429667
GCAAAAG93150.053.4501533
TACGCCC752.5491261E-548.341045145
CAAAAGC105050.047.6713074
CGACGAA7850.046.185715145
AGCAGGT35850.046.0948528
AAAGCAG129350.045.4940036
GTAGAAA17650.043.536381
GTTACGT1053.8289345E-641.4241031
TCTCGGG6000.041.08989145
Produced by FastQC (version 0.11.5)