FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11045185
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG143420.12984843621904024No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG135280.12247870904833191No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA121370.10988498608217065No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA26750.0119.205971
GTTACGT2050.074.239421
GAGCAAA233450.073.1082151
GCAAAAG237100.071.4222263
AGCAAAA256400.066.187362
GCAGGGT135700.056.8034069
GGATAGC20850.051.0878375
CGAAAGC68950.050.3393333
AGCAGGG222250.048.773398
GCAGGTA112250.048.3210339
CAAAAGC375100.045.8606154
TCGTACT10300.045.0245062
AAGCAGG459300.044.1076137
AAAGCAG459750.043.9678546
GGTCGTT11350.037.0523457
AAAAGCA466450.036.879235
GGTAGTT17500.036.4609687
GTAGTTT19150.033.6980558
ATTAGTA16900.031.3043921
CTATTCG14150.031.2603869
Produced by FastQC (version 0.11.5)