FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_NEGATIVE_CONTROL_P1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_NEGATIVE_CONTROL_P1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG70.684931506849315No Hit
GGATACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGG60.5870841487279843No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA50.4892367906066536No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT30.29354207436399216No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC30.29354207436399216No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC30.29354207436399216No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA30.29354207436399216No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG30.29354207436399216No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA30.29354207436399216No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA20.19569471624266144No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT20.19569471624266144No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC20.19569471624266144No Hit
GTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAAC20.19569471624266144No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA20.19569471624266144No Hit
CCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAGCAGACTCCACCCCAG20.19569471624266144No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA20.19569471624266144No Hit
GTATCCTCAACCCTGATCCTCTCACATTCACAGTCAATATCGTTTGTTTT20.19569471624266144No Hit
TAGTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTG20.19569471624266144No Hit
GTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTAGTGAGTATCT20.19569471624266144No Hit
AGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAA20.19569471624266144No Hit
CAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGA20.19569471624266144No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC20.19569471624266144No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA20.19569471624266144No Hit
GTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGC20.19569471624266144No Hit
ATCCAAACCAAAAGATAATAACCATTGGTTCGGTCTGTATGACAATTGGA20.19569471624266144No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA20.19569471624266144No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC20.19569471624266144No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA20.19569471624266144No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC20.19569471624266144No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC20.19569471624266144No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC20.19569471624266144No Hit
GAAGTAAACTGTAAATTCATAAACACAGAGACTAAACAACATTCTACTGA20.19569471624266144No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG20.19569471624266144No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA20.19569471624266144No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC20.19569471624266144No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA20.19569471624266144No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTCTTTCCTGCAAAGACACTTTCCA20.19569471624266144No Hit
GTTTGAGATATTCCCCAAGACAAGTTCATGGCCCAATCATGACTCGAACA20.19569471624266144No Hit
CCCTAGAACGTTCTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTC20.19569471624266144No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)