FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_DEMUXING_CONTROL_P1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_DEMUXING_CONTROL_P1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG140.5507474429583006No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA90.35405192761605037No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG70.2753737214791503No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA70.2753737214791503No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA70.2753737214791503No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT60.23603461841070023No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT60.23603461841070023No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT60.23603461841070023No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT60.23603461841070023No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT50.1966955153422502No Hit
CATATTGAGAGGGTCGGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT50.1966955153422502No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT50.1966955153422502No Hit
AAACTATTCTCTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCA50.1966955153422502No Hit
GTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATGAATCTATTCT40.15735641227380015No Hit
TCTCAATATGAGTGCAGACCGTGCTAGAAAAGTGAGATCTTCGAACTCAG40.15735641227380015No Hit
CTCTATTGTTAAGCTGTTTTGGATCAACCGTCCCTCATAATCACTGAGTT40.15735641227380015No Hit
GTTCACAGGTTGCACATACCAGGCCAAATGCCACTTCAGTGGTCACAGCC40.15735641227380015No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT40.15735641227380015No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT40.15735641227380015No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA40.15735641227380015No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC40.15735641227380015No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA40.15735641227380015No Hit
CCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAG40.15735641227380015No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA40.15735641227380015No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG40.15735641227380015No Hit
GTCCTACATTGTAGAAACACCAAACTCTGAGAATGGAATATGTTATCCAG30.11801730920535011No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAAC30.11801730920535011No Hit
GTCCGCAAACGAGTTGCAGACCAAGAACTAGGTGATGCCCCATTCCTTGA30.11801730920535011No Hit
TCCTTGTACTGTGGGGTATTCATCACCCGCCTAACAGTAAGGAACAACAG30.11801730920535011No Hit
ACCTAACTGACATGACTCTTGAGGAAATGTCAAGGGACTGGTCCATGCTC30.11801730920535011No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT30.11801730920535011No Hit
GTACAAGGACTTCTTTCCCTTTTTTGTTCACATAAGAATTTTTCAGCTTT30.11801730920535011No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT30.11801730920535011No Hit
GTTTTAGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAG30.11801730920535011No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC30.11801730920535011No Hit
GGTGTATATTCTGGTAAGGGAGACTGCTGTTTATAGCTCCCAGGGGTGTT30.11801730920535011No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT30.11801730920535011No Hit
CTTCTAACCGAGGTCGAAACGTACGTACTCTCTATCATCCCGTCAGGCCC30.11801730920535011No Hit
CTCTTAGATCTTCAAATGCGGCAGAATGGCATGCCATCCACACCAGTTGA30.11801730920535011No Hit
CTCAATATGAGTGCAGACCGTGCTAGAAAAGTGAGATCTTCGAACTCAGC30.11801730920535011No Hit
CTGCTGAGGATGTCAAAAATGCAGTTGGAGTCCTCATCGGGGGACTTGAA30.11801730920535011No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT30.11801730920535011No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC30.11801730920535011No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA30.11801730920535011No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA30.11801730920535011No Hit
AACATGGACAAAGCAGTTAAACTGTATAGGAAGCTCAAGAGGGAGATAAC30.11801730920535011No Hit
TCTTATTTCTTCAAACTTCTGACCTAATTGTTCCCGCCATTTCTCGTTTC30.11801730920535011No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG30.11801730920535011No Hit
GTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCCGCAAACGAGT30.11801730920535011No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC30.11801730920535011No Hit
ACCCTGACCAATCAGTCATTGCCACAACATCTTGCCTAACAGAGAACTTA30.11801730920535011No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC30.11801730920535011No Hit
TGTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCA30.11801730920535011No Hit
GCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCTTCAGTTTGTGT30.11801730920535011No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT30.11801730920535011No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA30.11801730920535011No Hit
CCTCTAGTGGAAAGCTTCCCTCTTGGGAGCACCTTCGTCCCTTTGATGAA30.11801730920535011No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT30.11801730920535011No Hit
ATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTGAG30.11801730920535011No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC30.11801730920535011No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC30.11801730920535011No Hit
GGACTCCAACTGCATTTTTGACATCCTCAGCAGTATGTCCTGGAAGAGAA30.11801730920535011No Hit
GACTTGAAGATGTCTTTGCAGGGAAGAACACTGATCTTGAGGTTCTCATG30.11801730920535011No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA30.11801730920535011No Hit
CCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTATTTAAAGCGACGGTAAAT30.11801730920535011No Hit
ATATAATGCAGAATTGTTAGTTCTACTGGAAAATGAAAGGACTCTGGATT30.11801730920535011No Hit
TCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACAATTGCTCCCTCTT30.11801730920535011No Hit
GCTAAGGCTATGGAGCAAATGGCTGGATCGAGTGAGCAAGCAGCAGAGGC30.11801730920535011No Hit
CTGTACCTGCGTCGCGTTACCTAACTGACATGACTCTTGAGGAAATGTCA30.11801730920535011No Hit
ATAGCGAGAGGATCACTTGAACCGTTGCATCTGCACCCCCATTCGTTTCT30.11801730920535011No Hit
ATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTTCT30.11801730920535011No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA30.11801730920535011No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers