FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_CW_C7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_CW_C7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6911196
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG527470.7632108827473566No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA401840.5814333727476402No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT347720.5031256529260637No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC254260.36789580269464217No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA231190.3345151837684823No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG228830.3311004347149176No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT198000.28649165788381636No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT184220.26655299603715477No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT165490.23945204274339782No Hit
GTAGTAAACATGACCAAAGAGTTCTTTGAGAACAAATCAGAAACATGGCC163180.2361096400680866No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA156260.22609690131780374No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC154330.22330433111721906No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA140320.20303287593059147No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT139930.20246857418021424No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA139910.20243963562891285No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT133630.19335293052027464No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT133570.19326611486637046No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA124460.18008460474858476No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT120700.1746441571039224No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT115930.16774231261853953No Hit
GTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAA111050.160681306100999No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG106130.1535624224808557No Hit
TGTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCA105800.15308493638438267No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT104060.15056728242116127No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC103730.15008979632468825No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC103350.1495399638499617No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC102600.14845476817615938No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG100560.14550303594341704No Hit
CCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAG99940.14460594085307377No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT99870.14450465592351888No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT95530.13822499029111604No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG93190.13483917978885276No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA91410.13226364872302854No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA88310.12777817327131225No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA87600.1267508547001127No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA84310.12199046301103311No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG83630.12100655226678567No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA82960.1200371107981889No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT82440.11928470846435263No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG81210.11750498755931679No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA80340.11624616057770609No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA77990.11284588079979212No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT76430.11058867379828326No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAAC74580.10791185780290415No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT74420.10768034939249299No Hit
GGTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGA74230.10740543315512974No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT73250.10598744414136135No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA72750.10526398035882648No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT72530.10494565629451111No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC71710.1037591756911539No Hit
CCTCAGCAGTATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACA71360.10325275104337947No Hit
GTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAAATCTGTGCTT71230.10306465045992039No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG71010.10274632639560505No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC70560.10209520899132364No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA70410.10187816985656319No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA69410.1004312422914934No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTCA100.00708874145.020682
AGGCGTA100.0070942626144.98291145
GAGCGAA38350.0140.14531
CTATAGT256.4630167E-6116.016553
GCAAAAG304550.0104.973923
AGCAGGC19300.0104.793348
TATAGTG301.5989996E-596.680444
ATTAGTA7000.093.254581
TAGGACA2050.091.964334
GCAGGGG64350.088.544349
GCAGGGT157850.087.5775769
TATTAGT2450.085.853411
TAGTATT1950.085.525013
AGCAGGG230300.084.830428
GAGCAAA385650.083.449121
AAGCAGG449900.083.061447
ACGGCCC353.44073E-582.84737145
CAAAAGC387850.082.6152344
AGCAAAA396050.081.344132
GGGTTAC1050.075.98521
Produced by FastQC (version 0.11.5)