FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_CW_B7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_CW_B7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4513891
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA482961.069941653442673No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT313360.6942125983990309No Hit
CCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAG294350.6520981565571699No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG262780.5821584969597183No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC211860.4693511651034551No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA200970.4452256379252401No Hit
GGCTAATGTGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGA197680.43793702594945244No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA187820.41609334385788227No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG180140.3990791979691136No Hit
TCCTCAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG179850.39843673673112623No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA162380.35973398560133596No Hit
GCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCA158540.3512269126569516No Hit
GTAGTAAACATGACCAAAGAGTTCTTTGAGAACAAATCAGAAACATGGCC149060.3302250763255028No Hit
TCCTCAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGGCCCA138090.3059223184609464No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG119320.2643395686781094No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA117210.25966510932585657No Hit
GAGATATGGGCCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGC107950.2391506573818464No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG106770.23653650475831162No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA106220.23531804378971488No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA95980.2126325159380233No Hit
GGCTGAGGAAAGGAGACGTGGTGTTGGTAATGAAACGGAAACGGGACTCT93880.20798021042156312No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT91490.20268544366711555No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT89850.1990522145971181No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT89810.1989635992539474No Hit
GAGGAAAGGAGACGTGGTGTTGGTAATGAAACGGAAACGGGACTCTAGCA87990.19493160113968191No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT84340.18684545107535827No Hit
GTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAA81730.18106329993347203No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC80570.17849345498152258No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT79230.17552484098530513No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG78990.17499314892628112No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT78850.17468299522518377No Hit
TGTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCA78630.1741956108377451No Hit
CTTAAGGCCACGAAGAGACTCACAGTTCTCGGAAAGGATGCTGGCACTTT72800.1612799245706199No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA72030.15957407921458447No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC71900.15928607934927982No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA70110.15532054274239232No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT68810.15244054408934554No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT67860.15033592968904214No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA66710.14778823857288534No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT63380.14041101125392705No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC62730.13897101192740366No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT61820.1369550128702709No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA61240.1356700903942962No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT57760.12796055553844787No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA57470.1273180943004605No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG56290.12470394167692575No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT54880.12158025083015961No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG54390.1204947128763189No Hit
GTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAAATCTGTGCTT53660.11887748286345418No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT53050.11752609888010145No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT52370.11601963804620005No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG51450.11398148515327462No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT50750.11243071664778789No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG49120.10881964141358309No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC48530.1075125651018157No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC48360.10713594989334035No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC47730.1057402582384023No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC46940.10399010521078156No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG46900.1039014898676109No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG46810.10370210534547687No Hit
CCTTTCCTCAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGC45830.10153102943779548No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA45480.10075564518505209No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA32000.0141.421221
GCAAAAG222950.0105.2397843
TAGTATT2250.099.89833
ATTAGTA6400.096.320551
AGCAGGC14700.096.156968
GAGCAAA248100.095.4413151
GCAGGGG33850.089.297829
TAGGACA900.088.619464
CAAAAGC266400.088.183994
GTATAGA259.376716E-487.028441
TCCGCCC259.395306E-486.98507145
GCAGGGT74850.086.868859
AAGCAGG325700.085.8633657
AGCAGGG114150.083.505168
TATTAGT1750.082.884241
TTAGTAG7450.082.7259142
AGCAAAA306250.077.06442
CGCTCCC1150.075.6592946
ACTAATA3050.073.675888
GCAAAGC1300.072.5068363
Produced by FastQC (version 0.11.5)