FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_CW_B6_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_CW_B6_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4972146
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT666811.3410909494612588No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC377960.7601546696335948No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG363970.7320179254591479No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA356690.7173763602275557No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA339530.6828640993245171No Hit
GTAGTAAACATGACCAAAGAGTTCTTTGAGAACAAATCAGAAACATGGCC262510.5279611660639089No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT240300.48329232488346074No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT215650.433716145905611No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA197630.39747424954938976No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA197440.39709212078647727No Hit
GTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAA194720.39162164586478354No Hit
TGTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCA183080.36821123112635873No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG166590.33504647691359024No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT142340.28627477954187186No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC128770.2589827410538629No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT122620.246613836359592No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT121490.24434117582227072No Hit
GTGCTGAGTCCTCTGTCAAAGAGAAAGACATGACCAAAGAGTTCTTTGAG117180.23567288651620447No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA116630.23456672430777373No Hit
CCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAG115970.23323932965765687No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT114110.22949849018914567No Hit
GGAACAGGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGA111150.22354532630377305No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA96220.19351805035491718No Hit
GGAACACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGG94050.18915373764165413No Hit
GTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATGAATCTATTCT90640.18229553194938364No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT89950.1809078011788069No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA89750.18050556037574117No Hit
GGTAGAAACTTTGGTTTCTCAGCCCCTGTAGTGTTGCAAATACTGTTTAC89050.17909771756501117No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA86120.17320488980009838No Hit
TCCTCAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG85640.17223951187274067No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT83780.16849867240422947No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG83120.1671712777541126No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT79300.15948847841555738No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC77060.15498338142122134No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT76770.15440013225677604No Hit
GTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAAATCTGTGCTT74220.14927156201768813No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT73720.14826596001002385No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT72730.14627486803484852No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC72380.14557094662948353No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA71780.14436422422028636No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT70090.14096528943438105No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA70000.14078428107300148No Hit
GCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCA69720.14022114394870946No Hit
TGTCTTTCTCTTTGACAGAGGACTCAGCACTGTCCAGGCCATTGTGCGAT68270.13730489812648303No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT67590.13593727939605957No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA66790.13432831618379668No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC63400.12751033457183278No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG62530.12576058707849688No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA62300.12529801015497133No Hit
CACATATACTATCTGGAAAAGGCCAATAAAATTAAATCTGAGAAAACACA61800.124292408147307No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA61060.12280411717596387No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC59260.1191839499483724No Hit
TCCTCAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGGCCCA59120.11890238138622639No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAAC59110.11888226934607311No Hit
GGCTAATGTGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGA58700.1180576756997884No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA58400.11745431449518981No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA57690.11602635964430649No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC57430.11550344660032107No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT56320.11327101014330632No Hit
CCTTTGGGGGACTCTCCAATGGGCCATGTTTCTGATTTGTTCTCAAAGAA56240.11311011382208006No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA56220.11306988974177348No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA55700.1120240636538026No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT54880.11037487636123317No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG54810.11023409208016015No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG54790.11019386799985358No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC52290.1051658579615321No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG52290.1051658579615321No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT51900.10438148839555395No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT51710.10399935963264152No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG51140.10285297334390421No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC51040.10265185294237135No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA50760.10208871581807936No Hit
GGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGA50550.10166636297486034No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT50330.10122389809148805No Hit
ACAAACACCGAAACTGGAGCACCGCAACTCAACCCGATTGATGGGCCACT50090.10074120912780919No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGGT100.0070888926145.019234
TCACTAG351.0913936E-11144.9784145
GAGCGAA43000.0142.67711
GCAAAAG312950.0108.2720643
AGCAGGC40550.0108.15288
ATTAGTA5000.0107.328261
GAGCAAA322300.0106.134831
GCAAAGC1150.0100.882943
CAAAAGC357800.094.862364
GCAGGGT105800.093.386379
AAGCAGG429300.093.224527
GCAGGGG48850.092.893019
TATTAGT1200.090.648871
AGCAGGG160600.089.8667458
TAGACCG259.384013E-487.0115365
TTAGTAG6350.084.499392
ACTAATA2150.084.289768
AGCGAAA73250.083.744892
AAAAGCA408300.083.129445
CGCTCCC1500.082.177566
Produced by FastQC (version 0.11.5)