FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_CW_B4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_CW_B4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5103622
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG245540.48110929845509715No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA152770.29933643204767124No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG129620.25397648963814323No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT116090.22746590558626795No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT98650.19329409584017No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA95550.18721997828209064No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC94340.18484911304167903No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT86210.1689192498974258No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA83550.16370726515404158No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG79630.15602644553221223No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT78050.15293060497035244No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT75840.14860034696927005No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA73810.14462277966510842No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT68840.13488459764457478No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT68780.13476703407893453No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT67690.1326312959698034No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA67380.1320238842139955No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT66050.12941789184230335No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG63590.12459778565105331No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA61490.12048306085364471No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA60540.1186216377310075No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC60380.11830813488930018No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA59170.11593726964888858No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT57070.11182254485148No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA56840.11137188451652572No Hit
CTGTTAACCTGCTCGAAGACAGCCACAACGGAAAACTATGTAGATTAAAA56270.11025503064294338No Hit
GAGTAGAGGCTTTGGGTCCGGCATCATCACCTCAAACGCATCAATGCATG55780.10929492819021472No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT53340.10451400985417808No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA52650.10316202884931526No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA51650.1012026360886445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA16700.0139.396581
GCAAAAG126100.0103.783373
AGCAGGC10100.0102.630918
CGTATAG301.5995853E-596.672835
TATTAGT1350.091.32271
CCGTATA406.118771E-790.6307754
GCAGGGG35900.085.612069
ATTAGTA3750.085.091261
GCAGGGT81200.083.378579
ACTAATA2700.083.226488
ACCGTAT353.4375444E-582.862433
AACCGTA451.2331475E-680.560692
AGCGAAA36800.079.812481
TACTAAT2900.077.486727
AGCAAAA172550.076.3914262
AGCAGGG133000.074.286138
CTACTAA3150.071.353756
AAGCAGG219350.070.124747
TCTACTA3350.069.258155
TAAACTA10450.068.688595
Produced by FastQC (version 0.11.5)