FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_800-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_800-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333357
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG116750.8756094579321216No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA99130.743461803552987No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA41460.31094448073546693No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG40480.3035946111956513No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT35170.26377031807685414No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT33050.24787060029684474No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30020.2251460036584351No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT28440.2132962139922016No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT26540.19904646692521208No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG23580.1768468609682178No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC22970.17227194217302644No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC22890.1716719528228374No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA22720.17039697545368568No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC21760.16319710325141729No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC20540.1540472656610345No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT20340.15254729228556194No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT20130.1509723202413157No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA20110.15082232290376846No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC19480.1460974067710298No Hit
GAATCTCCTTTTTTAACTAGCCATATTAAATTTTTGTAGAAGCTTTTTGC18570.13927252791262953No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC18300.13724756385574158No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT18280.1370975665181943No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA18090.13567259181149535No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG17650.13237265038545565No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC17130.12847271960922693No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG16920.12689774756498073No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT16920.12689774756498073No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC16910.1268227488962071No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT16330.12247282610733659No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG16050.12037286338167498No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT15940.11954787802516505No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC15190.11392297786714287No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA15110.11332298851695383No Hit
GAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA15080.11309799251063293No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG14840.11129802446006583No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG14780.11084803244742407No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT14690.11017304442846139No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT14670.11002304709091414No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC14570.10927306040317784No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC14540.10904806439685695No Hit
AGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGA14520.1088980670593097No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT14110.1058231216395909No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG14040.10529813095817549No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA14040.10529813095817549No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT13900.10424814959534467No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT13860.10394815492025017No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCACG100.007084859145.042881
GGTATAC100.0070943995144.977628
GAGCGAA7450.0140.175671
GCAGGTA19250.091.881919
GAGCAAA70100.083.9014051
GCAGGGT36350.077.773419
AAGCAGG88900.077.7073447
CACCTAC3050.076.073793
AGCGAAA13850.075.387212
CCACCTA3100.072.507842
AGCAAAA81250.072.1062552
CCTACTA3400.070.375255
GCAAAAG83550.070.03453
GTTATGT851.6916601E-1068.255471
GCGAAAG15300.068.242673
CGAAAGC15450.067.5801244
TTATAGG2050.067.2151
GTAGAAA9200.064.638681
AGCAGGG54950.064.243958
AAAGCAG109600.063.37826
Produced by FastQC (version 0.11.5)