FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_795-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_795-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737308
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG69170.3981447158477368No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA43880.25257467299983655No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA43590.2509054237935933No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT37390.21521802697046233No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC27000.15541285713298966No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA23490.135209185705701No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG22260.12812926665853147No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC21750.1251936904682417No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT20720.11926497777020541No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT20430.11759572856396218No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT19040.10959484443748604No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA18520.10660170792973958No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA18510.10654414761228292No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC17920.10314808888233981No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7500.0131.493421
TACCGGA301.5988628E-596.677992
GTTACGT2000.083.391971
ATACCGG353.4345234E-582.874011
GAGCAAA63300.079.7318651
TTACGTG2100.079.414072
AGCGAAA13600.071.975342
TACGTGT2350.070.965763
AAGCAGG87500.068.5979467
GCGAAAG14750.067.8384553
CGAAAGC14500.067.007854
GCAGGTA17150.066.7854469
AGCAGGT36050.059.9238748
AGTCGGC851.304943E-859.712885
AAAGCAG111100.054.1692626
CAAAAGC95650.053.2158474
AGCAAAA95000.053.045692
GGTTATA1152.4811015E-950.445051
GCAAAAG100150.050.1007233
TCACGAT752.5496009E-548.3389973
Produced by FastQC (version 0.11.5)