FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_740-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_740-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187036
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG14520.12232147971923345No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC13660.11507654359261218No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12970.10926374600264861No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC12080.10176607954602893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5500.068.561051
AGCGAAA7300.049.6646462
GCGAAAG7950.047.4281773
CGAAAGC7750.046.7808953
GTTACGT2250.045.1213761
GAGCAAA33700.044.7579541
TACGTGT2450.041.4345053
GTTATGT7600.041.0290681
GATATAC4150.040.186483
AGACGAC2400.039.276463
TTACGTG2600.039.0440522
CTACTGA15450.038.95386
CAAAAGC39650.037.489614
GTCGTCT1406.616665E-736.246033145
GCAAAAG41050.035.6811183
ACTCCGA3050.035.6638531
ACGCTAG1051.8523647E-434.5287555
CATTCTA13150.034.1874052
TAGTCGG1707.8323865E-834.1139078
AAAGCAG55200.033.7593656
Produced by FastQC (version 0.11.5)