FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_644-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_644-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140183
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7200.069.500851
GAGCAAA34100.066.780361
GCGAAAG11150.056.5772252
GCAAAAG41400.055.5208553
CAAAAGC41700.054.773664
AGCGAAA11300.054.552411
TCGTCGA400.006043639554.3919641
AGCAAAA44150.051.569892
CGAAAGC12400.050.289123
AAAGCAG65900.042.141566
AAAAGCA57850.039.4824835
AAGCAGG90150.029.6717827
GCAGGGG32600.027.573199
GTGATCG1450.001227455124.9968329
GATATAC4154.3655746E-1122.7139423
GTATAGC3304.675894E-821.9765511
GTATAAG3706.6283974E-921.5607781
GTATATA11250.021.27331
GTATTAT14100.021.0881371
CGAGGTG3508.726238E-820.7171135
Produced by FastQC (version 0.11.5)