FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_644-2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_644-2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA70.6809338521400778No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA60.5836575875486382No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG40.38910505836575876No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG40.38910505836575876No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT40.38910505836575876No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT30.2918287937743191No Hit
CTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAA30.2918287937743191No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA30.2918287937743191No Hit
CTGTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACT30.2918287937743191No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC30.2918287937743191No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT30.2918287937743191TruSeq Adapter, Index 10 (95% over 21bp)
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG30.2918287937743191No Hit
TATAACTTCTGTTCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA30.2918287937743191TruSeq Adapter, Index 18 (96% over 30bp)
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT30.2918287937743191No Hit
GTCAACATCCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGG20.19455252918287938No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT20.19455252918287938No Hit
ATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAG20.19455252918287938No Hit
CCTCTCGTCATTGCAGCAAATATCATTGGGATCTTGCACCTGATATTGTG20.19455252918287938No Hit
GTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTCTGCATTGTCT20.19455252918287938No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA20.19455252918287938No Hit
CTCCCCGCCCCTGGAAGGACAAATCTTCTGGCTTTGCACTTTCCATCATT20.19455252918287938No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT20.19455252918287938No Hit
GTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTAGTGAGTATCT20.19455252918287938No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT20.19455252918287938TruSeq Adapter, Index 10 (96% over 26bp)
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC20.19455252918287938No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT20.19455252918287938No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC20.19455252918287938No Hit
GTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTT20.19455252918287938No Hit
CATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTT20.19455252918287938No Hit
TATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCCA20.19455252918287938No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC20.19455252918287938No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTC20.19455252918287938No Hit
TTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCC20.19455252918287938No Hit
AAATTGAGACTGGCCACAGGATTGAGGAATATCCCGTCTATTCAATCTAG20.19455252918287938No Hit
AGTTATCCCTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAG20.19455252918287938No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA20.19455252918287938No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC20.19455252918287938No Hit
GGCACGGTGAGCGTGAACACAAATCCTAAAATTCCCTTAGTCAGAGGTGA20.19455252918287938No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG20.19455252918287938No Hit
ACCCAGGAGATTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCA20.19455252918287938No Hit
CTCAAGGCACCAAACGATCATATGAACAAATGGAGACTGGTGGGGAGCGC20.19455252918287938No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA20.19455252918287938No Hit
TTTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTT20.19455252918287938No Hit
ATTACATCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTTAG20.19455252918287938No Hit
GACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAA20.19455252918287938No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT20.19455252918287938No Hit
TTCCTCAACTGTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCT20.19455252918287938No Hit
GCTCTATGTTGACGATGGTCATTTTGTCAACATCTGTCTCTTATACACAT20.19455252918287938No Hit
TACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTG20.19455252918287938No Hit
ATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAGGATCA20.19455252918287938No Hit
CATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAG20.19455252918287938No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers