FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_562-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_562-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823486
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG42370.2323571445023433No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA30460.16704268637104974No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC24320.13337091702376658No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT20400.11187363105611997No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC18530.10161854820930898No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC18530.10161854820930898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5300.0108.094951
AGCGAAA9650.065.380181
GCAGGTA10400.060.638659
GCGAAAG10850.059.471272
CGAAAGC11300.056.4613383
GTTACGT1850.054.8795471
GAGCAAA53500.049.7469521
TTACGTG2100.048.3343282
GCGATTA901.325745E-648.325054145
GTTATGT7000.046.619611
GCTATAC2500.046.4009553
GGTTATA2050.045.9879071
TACGTGT2300.044.1313443
CGAAGCG2200.042.8417935
AAGCAGG74700.042.7938737
AGTCGGC1401.41535565E-841.4294245
ACTCCGA3200.040.792161
AGCAAAA65350.039.939612
CGGTAAC2200.039.5560341
GCAAAAG67400.038.83243
Produced by FastQC (version 0.11.5)