FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_559-3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_559-3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505870
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC17910.11893456938513948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA44400.061.2621231
GCGAAAG12450.052.999472
CGAAAGC12950.050.953163
GAGCGAA12650.050.4586831
CAAAAGC54700.048.91464
CGACGAT604.9424334E-448.3401764
GTTACGT2300.047.305011
GCAAAAG57000.046.8136443
AGCGAAA13850.046.6105231
GTAACGG2200.046.1582261
AGCAAAA61500.044.0956732
TTACGTG2400.042.2976532
TCGACGA700.001056075641.4344373
AAAGCAG79250.040.990036
GCGTACA850.002740521234.122482
AAGCAGG94550.033.580127
ACTCCGA3250.033.4773941
AAAAGCA83950.031.9580655
CGGGTTG3000.031.4211145
TAACGGG3000.031.4211142
Produced by FastQC (version 0.11.5)