FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_548-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_548-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1692553
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG155430.9183168857932366No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA140540.8303432743317343No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG50190.29653428873423754No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT45400.26823384555756896No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA41690.24631429562323898No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC39970.2361521323113663No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT38010.2245719927234184No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT37610.22220869892995965No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG34500.20383408968581782No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA33790.19963924320242854No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC31770.1877046095454618No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC30360.17937399892351968No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC29230.1726976939569987No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT28680.16944816499099288No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT27660.16342176581767306No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA26960.15928600167912024No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG26170.15461849643703918No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC25800.15243244967808983No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT25620.1513689674710334No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC25260.14924200305692054No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC24780.14640605050477No Hit
GAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA24260.14333376857327362No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC24190.14292019215941834No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT22840.13494407560649505No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG22770.13453049919263976No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT22570.13334885229591037No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC22560.13328976995107392No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA22440.13258078181303629No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT22280.13163546429565276No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT21910.12944941753670341No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC21480.12690887670873527No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT21420.12655438263971644No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC21320.12596355919135177No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG20630.1218868773976354No Hit
ATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTT20610.12176871270796247No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG19720.11651038401751673No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC19670.1162149722933344No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG19650.11609680760366145No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG19550.11550598415529677No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA18980.11213829049961804No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT18810.11113389063739805No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG18780.11095664360288866No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC18710.11054306718903337No Hit
CATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAG18370.10853426746459344No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCC18330.10829793808524756No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA17860.1055210678779335No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTCTTTCCTGCAAAGACACTTTCCA17840.10540290318826057No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA17830.10534382084342411No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA17430.10298052704996534No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG17200.10162163311872657No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG17160.10138530373938069No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG17080.10091264498068894No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACTA100.007094234144.97989145
GAGCGAA10150.0142.900631
GCGTCAG256.4563974E-6116.0353241
TCCCGGA301.5994754E-596.670382
GCAGGTA25100.091.839849
GCAGGGT44150.089.319439
GAGCAAA86450.083.805161
AGCGAAA17300.083.399162
CGTCAGA353.4373094E-582.860342
TTATAGG1250.081.224731
GCGAAAG18550.077.7792743
CGAAAGC18650.077.362234
AAGCAGG113400.076.261467
AGCAAAA96750.074.713472
AGCAGGG65500.073.153988
GTCAGAT406.663246E-572.502793
GCAAAAG101750.070.757033
GTAGAAA10500.066.30591
TCCCCCC902.8194336E-1064.43559
AAAGCAG136650.063.562646
Produced by FastQC (version 0.11.5)